use of glycoside hydrolase family proteins in cellulose processing.

摘要

use of glycoside hydrolase family proteins in cellulose processing. The present invention provides recombinant gh61 proteins obtained from myceliophtora thermophila and nucleic acids encoding such proteins. The present invention also provides protein fractions isolated from the supernatant of m. thermophila with gh61 protein activity. Such preparations may be used to increase the yield of reaction products in which a cellulose-containing substrate is subjected to saccharification by one or more cellulase enzymes, such as endoglucanase, β-glycosidase or cellobiohydrolase. Combinations of gh61 protein proteins and cellulases can be used to break down cellulosic biomass into fermentable sugars in ethanol production.