PROTEASE (METALOPROTEASE PR4A3) OBTAINED FROM MANGUEZAL SEDIMENT METAGENOMA AND METALOPROTEASE USED BY METAGENOMIC DEOXYRIBONUCLEIC ACID

摘要

protease (metalloprotease pr4a3) obtained from the mangrove sediment metagenome and use metalloprotease obtained from metagenomic dna (deoxyribonucleic acid) from the mangrove sediment The present invention relates to the use of a protease, which we call metalloprotease pr4a3 isolated from mangrove sediment metagenome, and its use in antitumor, antifungal, antiviral and antiparasitic treatment. In order to obtain metalloprotease we used gel filtration chromatography using the hiprep 16/60 sephacryl s 100 column under specific conditions. Activity assays have shown that the molecule has 60 kda subunits and is functional after subsequent purification steps. pr4a3 activity remained optimal in thermal treatments up to 60 ° c and optimal ph of 8.5, and in the range of ph 9 - 12 the activity is above 80%. It has moderate thermotolerance and thermostability at temperatures of 40 and 50 ° C, desirable characteristics in biotechnological applications. mass spectrometry analysis of pr4a3 identified it as belonging to the peptidase family (m42) showing a high similarity to human gut metagenome glutamyl aminopeptidase, exhibiting several unique biochemical properties. In view of the strong evidence that it is a molecule with therapeutic potential, we believe that metalloprotease pr4a3 can have healing properties against tumors, mycoses, viruses and protozoans, as well as other diseases, pathogens or diseases, provided they are tested within pharmacological criteria.