Method for stimulating plants of the family Leguminosae (Fabaceae) to produce biflavanu00f3ides

摘要

Method for stimulating plants of the family Leguminosae (Fabaceae) to produce biflavonu00f3ides the present invention relates to a protocol of germination and multiplication in vitro of plants of the family legUminosae (Fabaceae) particularly of the genre poincianella and more particularly of the species poincianella pyramidalis Tull with the best parameters for the increase in the concentration of biflavonoides AGAtisflavona and amentoflavona which possess cytotoxic activity.More particularly, the present invention relates to a method for obtaining an extract concentrate in a mixture of biflavonu00f3ides in which major components are agatisflavona and amentoflAVONA. The present invention also refers to a method for obtaining isolated from these active compounds.The present invention also refers to the use of the said extract or active substances isolated from fraction in the formulation of a chemotherapeutic composition for the treatment of cancer in animals and humans.The invention has the potential application in biotechnology industry whose products could be used in the industry of agents in the manufacture of anticancer drugs for use in humans and animals.