Ionic liquids commuted for pre-treatment of biomass and enzyme hydrolysis

摘要

The present invention relates in one aspect, a method for preparing a sugar composition.The method includes: forming a mixture including biomass, polysaccharide and an ionic liquid solution, in which the ionic liquid solution containing water and an ionic liquid.And in the ionic liquid contains an anion of acid acid and a cation.The pH of the mixture is greater than or equal to about 10, and the molar ratio of acid to the acid anion cation is at least about 1: 2.The method further includes: keeping the mixture under conditions sufficient to dissolve at least a portion of the polysaccharide present in the biomass of polysaccharide; reducing the pH of the mixture containing the.Issacaru00eddeo dissolved until at least about 7; adding at least one of glycoside hydrolase to the mixture having the lowered pH; and maintaining the mixture containing the glycoside hydrolase of conditions sufAdequate to hydrolyze at least a portion of the polysaccharide is dissolved, thus forming the sugar composition. The sugar composition contains at least one monossacaru00eddeo or oligosaccharides.The method may also include the fermentation of the sugar composition.