nucleic acid, expression cassette, cloning vector or vehicle, transformed or transduced cell, transgenic plant, plant or seed cell, polypeptide, composition, antibody, hybridoma, method for isolating or identifying a polypeptide, producing a recombinant polypeptide, producing a compound, producing or making a butadiene, a dialkene or a compound, producing a polymer, resin or article of manufacture, enzymatically catalyzing the conversion of a crotyl alcohol to a 3-buten-2-ol, enzymatically catalyzing the conversion of a 3 -buten-2-ol to a butadiene, enzymatically catalyze the conversion of a crotyl alcohol to a butadiene, peptide or polypeptide, and use of or a method for use of a polypeptide.

摘要

in alternative embodiments, unnaturally or genetically engineered vinyl isomerase dehydratase enzymes are provided, including alkenol dehydratases, linalool dehydratases and crotyl alcohol dehydratases. in alternative embodiments, unnatural or genetically engineered polypeptides are provided having an activity comprising, for example, a vinyl isomerase dehydratase, an alkenol dehydratase, a linalool dehydratase and / or a combination of crotyl alcohol dehydratase activity or a combination thereof. . in alternative embodiments, unnaturally or genetically engineered nucleic acids (polynucleotides) encoding polypeptides described herein are also provided, expression or cloning vehicles comprising or having nucleic acids contained herein as described herein and unnatural or genetically engineered cells comprising or having nucleic acids contained herein as described herein. In alternative embodiments, methods are also provided for making various organic compounds, including methyl vinyl carbinol and butadiene.