DIAGNOSTIC METHOD AND MONITORING OF CHRONIC TRYANOSOMIASE TREATMENT MONITORING, CHRONIC DIAGNOSTIC AND MONITORING METHOD OF TREATMENT OF CHRONIC TRIPANOSOMIAS AND DIAGNOSTIC DIAGNOSIS MONITORING FOR CHRONIC TRIPANOSOMIASIS

摘要

Diagnostic and monitoring method for the treatment of chronic trypanosomiasis, Diagnostic and monitoring method for the treatment of chronic chagas disease, and Diagnostic kit for use in the diagnosis and monitoring of the treatment of chronic trypanosomiasis and chagas disease refers to the diagnosis and monitoring of trypanosomiasis treatment in order to identify the stage of the disease and make it possible to choose the treatment regimen to cure protozoan infected individuals of the trypanosomatidae family suffering from chronic disease. In the case of nasturtium disease, the protozoan is t. I crossed. The diagnostic and monitoring method for the treatment of chronic trypanosomiasis, including chronic chagas disease, comprises the steps of: (i) determining the specific markers of the trypanosomatidae protozoan kdna integration into the host genome; (ii) extract the genomic DNA from an infected individual's sample; (iii) cleaving the genomic DNA with specific restriction enzymes and separating the resulting fragment mixture; (iv) subjecting the fragments to purification and southern blot analysis (v) hybridizing the fragments with a radiolabeled complementary (kcr) probe and (vi) performing comparative profile analysis of genomic DNA fragments hybridized to specific integration markers of the parasite kdna of the trypanosomatidae family. The invention further includes a diagnosis and monitoring kit for the treatment of chronic trypanosomiasis, including chronic chagas disease, comprising: (i) specific gene markers of t kdna integration. cruzi in the host genome; (ii) DNA extraction reagents and additives and analysis of fragments resulting from cleavage with specific restriction enzymes; and (iii) instructions for the extraction and analysis of the fragments.