Device for lateral flow immunoassay device, forward flow immunoassay method to detect antibodies antilipoidais in a subject.Method for diagnosing syphilis in a subject and methods to immobilize cardiolipin imunorreativa on a microporous substrate.

摘要

Device and method for immunoassay imunorreativa immobilize cardiolipin.The present invention relates to compositions, methods and devices for the detection of antibodies antilipoidais and the diagnosis of the disease, for example, syphilis is described.In particular, described a method to immobilize an antigen lipoidal comprising cardiolipin, lecithin and cholesterol, on a solid support (such as a nitrocellulose membrane).The ability to immobilize an antigen on a membrane lipoidal satisfies the need for a long time, existing test based on the membrane for detection of antibodies antilipoidais.Also described are immunoassay devices to perform both tests treponemais and not treponemais for syphilis.