NEURAL STAMCELLE WITH INCREASED PASSAGE EQUIPMENT, PROCEDURE FOR MANUFACTURE OF THE NEURAL STAMCELLE WITH INCREASED PASSENGER AND METHOD OF EQUIPMENT OF THE NEURAL STAMCELLE TO INCREASE THE PASSWORD OF THE NEURAL STAMCELLE

摘要

The object of the present invention is to provide a neural stem cell having increased passage ability, a method for manufacturing a neural stem cell having said increased passage ability, and others. The present invention, in one embodiment, provides a neural stem cell having increased passage ability having the following characteristics: (a) the N-type calcium channel gene is knocked out or knocked down in said cell, (b) the influx of Ca2+via the N-type calcium channel is substantially absent or suppressed in said cell, (c) said cell can be passaged for at least 4 generations (more preferably 15 generations) or more, and (d) said cell maintains the differentiation potential into a nerve cell even after passage for 4 generations (more preferably 15 generations)).