NEURAL STEM CELL HAVING INCREASED PASSAGE ABILITY, METHOD FOR MANUFACTURING NEURAL STEM CELL HAVING SAID INCREASED PASSAGE ABIILITY, AND METHOD FOR CULTURING NEURAL STEM CELLS FOR INCREASING PASSAGE ABILITY OF NEURAL STEM CELLS

摘要

The object of the present invention is to provide a neural stem cell having increased passage ability, a method for manufacturing a neural stem cell having said increased passage ability, and others. The present invention, in one embodiment, provides a neural stem cell having increased passage ability having the following characteristics:(a) the N-type calcium channel gene is knocked out or knocked down in said cell,(b) the influx of Ca2+ via the N-type calcium channel is substantially absent or suppressed in said cell,(c) said cell can be passaged for at least 4 generations (more preferably 15 generations) or more, and(d) said cell maintains the differentiation potential into a nerve cell even after passage for 4 generations (more preferably 15 generations).