method of determination of alkaline phosphatase activity in biological fluids

摘要

the invention belongs to the field of medicine, namely to clinical laboratory diagnosis, and can be used to determine the activity of alkaline phosphate u0437u044b in biological fluidsin particular, in saliva.the essence of the invention is a reagent to the sample receiving the biological fluid.spend u0438u043du043au0443u0431u0430u0446u0438u044e obtained mixture with cocktail and determine the concentration of the protein after incubation by measuring the optical density of the mixture on the x u043eu043bu043eu0441u0442u043eu0439 samples and subsequent calculationwith the receipt of the original mixture as a biological fluid using saliva, and reagent prepared by mixing buffer solution u0434u0438u044du0442u0430u043du043eu043b amin 1 mmol / ml with magnesium with 0.5 u043cu043au043cu043eu043bu044c / ml and an r - u043du0438u0442u0440u043eu0444u0435u043du0438u043bu0444u043eu0441u0444u0430u0442u0430 5 u043cu043au043cu043eu043bu044c / ml followed by 1 ml to obtain a mixture to u0440u0435u0430u0433u0435u043du0442u0443 add 100 mkl samples of biological fluidsafter stirring the obtained mixture u0438u043du043au0443u0431u0430u0446u0438u044e are within 5 min at a temperature 37u00b0u0441, add in 1 ml of solution of sodium u0433u0438u0434u0440u043eu043eu043au0438u0441u0438 05 mol / l (20 g / l) and mix with subsequent measurement of optical density against a single sample with the wavelength of 405 nm in a ditch with a layer thick 10 mm during the time jeni to 30 minutesand the activity of alkaline phosphatase in the e / l count using the importance factor, calculated as the ratio of total solution in the ditch to the coefficient u043cu0438u043bu043bu0438u043cu043e used as p - u043du0438u0442u0440u043eu0444u0435u043du043eu043bu0430 1875 and the volume of biological fluid sample, the sample is in the form of a mixture u0445u043eu043bu043eu0441u0442u0443u044e reagent with distilled water in the same numbers as the original u0441u043cu0435u0441 and, thewith the above same operations as the original mixture.