A mixotrophic culture process for generation of biodiesel and bioethanol with remediation of phenol polluted industrial waste water.

摘要

The present invention is related to an environmentally sustainable process for producing biofuels coupled to phenol bioremediation. The invention provides a method for generation of biofuels while remediating toxic industrial pollutant phenol. The xenobiotic phenol originates in wastewater of various industries as petrochemical, pharmaceuticals, coking plants, pesticides, leather industries etc. In accordance with this invention, the xenobiotic phenol is used as a microalgal carbon source thus leading to enhanced production of biomass with high neutral lipid content which could be exploited as biodiesel feedstock. The lipid extracted biomass was further employed as a substrate for bioethanol fermentation. Thus, the present invention is an environmentally sustainable process as it will cause environmental protection by remediation of toxic waste phenol along with generation of cost effective biodiesel and bioethanol from the spent biomass.