SYSTEM AND METHOD FOR MEASURING SULPHITE IN FOOD SAMPLES USING AN AMPEROMETRIC BIOSENSOR AND THE USE OF SAID BIOSENSOR

摘要

The invention relates to a system for measuring sulphite in food samples using a biosensor a method for determining sulphite in food samples using said biosensor and the use of said biosensor for measuring the sulphite values in food samples. The system uses an amperometric biosensor comprising a bi enzymatic system a working electrode a counter electrode and a reference electrode. The bi enzymatic system is composed of the human sulphite oxidase and peroxidase enzymes and the immobilisation of the enzymes in the working electrode is carried out via simple physical retention. The mixture of the enzymes is mixed with a chemical mediator. The method comprises the following phases: applying a sample to the amperometric biosensor; changing the electrical current upon coming into contact with the analyte to be detected; and amperometrically measuring said electrical current in solutions with continuous agitation using a constant potential.