System for measuring sulphite in food samples using biosensor; method for the determination of sulfit in food samples using the quoted biosensor; and use of the quotation biosensor to measure the sulfit values in food samples (Machine-translation by Google Translate, not legally binding)

摘要

System to measure sulfite in food samples by means of biosensor; method for the determination of sulfite in food samples using said biosensor; and use of said biosensor to measure sulfite values in food samples. The system uses an amperometric biosensor comprising a bi-enzymatic system; a working electrode; a counter-electrode; and a reference electrode. The bi-enzymatic system is composed of human sulfite oxidase and peroxidase enzymes; and the immobilization of the enzymes in the working electrode is carried out by simple physical retention. The mixture of the enzymes is mixed with a chemical mediator. The method comprises the phases of application of a sample to the amperometric biosensor; change of electric current upon contact with the analyte to be detected; and measuring said electric current amperometrically in solutions with continuous agitation, using a constant potential. (Machine-translation by Google Translate, not legally binding)