SCAR PRIMERS AND A KIT FOR THE AUTHENTICATION OF UNANI DRUG AMLA (EMBLICA OFFICINALIS) AND ITS ADULTERANT SHAKARKAND (IPOMEAE BATATAS)

摘要

This invention relates to SCAR Primers and a kit for the authentication of unani drug Amla (Emblica officinalis) and its adulterant shakarkand (I pomeae batatas) 1 (a) Genuine drug ,Amla (Emblica officinalis)5-AGGGTTGTTTCCCCTGATTT-3", 5"-TTTATGCTTCCGGCTCGTAT-3" and its adulterant 1(b)Shakarkand (Ipomeae batatas) 5"-ACCTAGAAGGCGGCCGCGAA-3", 5"-TGCGAAAATCCCTGTTTTCTAAGGTGT-3". and A kit for authentication of genuine drug which comprises: kit (c) an oligonucleotide primer set genuine drug Emblica officinalis comprises a forward oligonucleotide primer having the nucleotide sequence set forth in SEQ ID NO: 15 (5"-AGGGTTGTTTCCCCTGATTT-3") and a reverse oligonucleotide primer having the nucleotide sequence set forth in SEQ ID NO: 16 (5"- TTTATGCTTCCGGCTCGTAT-3") (d) an oligonucleotide primer set for the adulterant Ipomeae batatas that comprises a forward oligonucleotide primer having the nucleotide sequence set forth in SEQ ID NO:17 (5"-ACCTAGAAGGCGGCCGCGAA-3") and a reverse oligonucleotide primer having the nucleotide sequence set forth in SEQ ID NO: 18 (5"-TGCGAAAATCCCTGTTTTCTAAGGTGT-3").[IN-DEL-2010-01890A]