Markers and their ratio to determine the risk for early-onset preeclampsia

摘要

As indicated above, both GBP-1 and ESM-1 play a role in the regulation of angiogenesis and are released by activated endothelial cells. For ESM-1 we have shown the concentrations at different time points during gestation. Here it was tested whether GBP-1 is increased in preeclampsia (PE) and whether the ratio between GBP- 1 and ESM -1 is informative during gestation. Plasma samples from high risk pregnancies divided in 23 healthy, 11 severe early-onset PE and 7 severe late-onset PE pregnancies were collected at regular intervals between week 12±2 and birth. GBP-1 and ESM-1 were both measured by ELISA. Surprisingly, the ratio between GBP-1 and ESM-1 differed between the three groups at week 12±2. Although this ratio was comparable for women with healthy pregnancies and those that developed severe lateonset PE, the GBP-1/ESM-1 ratio was significantly increased between women that develop severe early-onset PE and healthy control pregnancies. This indicates that the ratio between GBP-1 and ESM-1 is a prognostic parameter to identify the women at risk for severe early-onset PE already as early as 12±2 weeks of gestation. This ratio serves, therefore, both as an important rule-in and rule-out parameter. The increased GBP-1/ESM-1 ratio that as observed in women that will develop early- onset preeclampsia is probably caused by endothelial cell activation in these conditions.