Process for the preparation of modified oligonucleotides having at least one phosphodiester bond, wherein the non-bridging oxygen atom is replaced by a sulfur atom

摘要

method of production is that it is the condensation osadzaju0105c in first cycle nukleozyd with prolonged chain on solid medium, and added successively, in the presence of a catalyst, 2 - tiono - 1,3,2 - oksatiafosflanowe nucleotide derivatives and amidofosforynowe derivatives nukleoty class, alternately, in the form of the same or different form, or mixtures thereof,and after each stage of condensation using derivative amidofosforynowej, apply reagent oxidation with peroxides and organic group selected wodoronadtlenku00f3w and repeats every cycle reaction, until the manufacture oligodeoksyrybonukleotydu of desired sequence and the horse figuracjach at internukleotydowym tiofosforanowym phosphorus atom.after completion of the synthesis of obtained product, whether or not the blocking group and medium.