Detection of non-small chromosomal abnormalities associated with cancer prognosis cell lung

摘要

A method for predicting the prognosis of disease in a patient who is being treated for lung cancer, from a biological sample of the patient, the method comprising: contacting the sample with two or more probes, each probe targeted to a subregion different chromosome, wherein the chromosome 1p13.3 include subregions and at least one chromosomal subregion selected from the group consisting of: 19q12, 19q13.11-13.12, 17q25.1, 6q13, 12p13.3, 11q13.1, 19q13.33 -13.43, 6p21.2, 11q12.2-13.1, 17q22, 17q21.32-21.33, 2q22.3-2q23.3, 6q22.31-23.3, 8p23.1, 2q24.2, 2q33.1-2q33.2 , 6p21.31, 2q33.3, 9p13.2, 4q24, 5q11.2, 16q12.1, 5q11.2, 5q15, 5q12.1-13.1, 14q22.1-23.1, 14q23.2-24.1, 9p35.3 , 4p13, 5q12.1, 3q13.3, 4p12, 14q21.1, 4p13, 2q34, 14q21.3, 14q12, 3q11.2, 14q22.3-23.1, 14q22.1, 4q21.21-4q22, 10q11.23 -21.1, 5q11.2, and 5q13.1-13.2; incubating the two or more probes with the sample under conditions in which each probe binds selectively with a polynucleotide sequence in its chromosome or chromosomal region target to form a stable hybridization complex; and detecting hybridization of two or more probes, wherein a hybridization pattern showing at least a gain or loss in a chromosomal region chosen as the target by the probes is indicative of an increased risk of bad prognosis of disease when compared with a reference measurement prognostic disease in patients who have no gain or loss in the two or more chromosomal regions selected as the target by two or more probes, wherein the biological sample comprises a tumor sample, wherein the cancer is cancer of non-small cell lung early stage, and where the patient has been treated with surgical resection without any monitoring or neoadjuvant chemotherapy.