Analysis of protein expression to identify genotoxic compounds

摘要

A method of high throughput screening of compounds for their genotoxic activity and / or progenotóxica comprising the step of determining the expression levels of active forms of at least proteins: antigen cell tumors p53 (p-p53 (Ser15) ) inhibits cyclin dependent kinase 1 (p21), histone H2A.X (p-H2A.X (Ser139)), ataxia telangiectasia mutated protein serine kinase (p-ATM (Ser1981)) and serine / threonine protein point kinase 1 (p-Chk1 (Ser345)) control in an incubated with compounds compared to expression levels in a system that was incubated with the compounds, where an increase by a factor of at least one system, 5 in the expression levels of these proteins in incubated with the compounds compared to the system not incubated with the compounds of this system is indicative activity sensitivity and / or specificity of at least 80%.