MUTANT PYRROLYSYL-TRNA SYNTHETASE, AND METHOD FOR PRODUCTION OF PROTEIN HAVING NON-NATURAL AMINO ACID INTEGRATED THEREIN BY USING THE SAME

摘要

Disclosed is a method for introducing a lysine derivative (particularly an N E-benzyloxycarbonyl-lysine (Z-Lys)derivative) having a useful functional group such as a heavy atom, selenium, areactive functional group, a fluorescent group ora crosslinker, which is suitable as a non-natural amino acid, into a desiredprotein in a site-specific manner. Also disclosed is amutant pyrrolysyl-tRNA shnthetase which has the substitution of at least oneamino acid residue selected from a tyrosine residueat position-306, a leucine residue at position-309 and a cysteine residue atposition-348 each constituting a pyrrolidin-binding sitein the amino acid sequence for pyrrolysyl-tRNA shnthetase depicted in SEQ IDNO:2, wherein the substitution of the amino acidresidue is the substitution of a tyrosine residue at position-306 by a glycineor alanine residue, the substitution of a leucine residueat position-309 by a glycine or alanine residue, or the substitution of acysteine residue at position-348 by a valine, serine or alanineresidue.