IMPROVED OPTIMIZED STRAINS OF YARROWIA LIPOLYTICA FOR HIGH EICOSAPENTAENOIC ACID PRODUCTION

摘要

Described are engineered strains of the oleaginous yeast Yarrowia lipolyticacapable of producing an oil comprisinggreater than 50 weight percent of eicosapentaenoic acid ["EPA"], an .omega.-3polyunsaturated fatty acid, measured as a weightpercent of total fatty acids ["% TFAs"] and having a ratio of at least 3.1 ofEPA % TFAs, to linoleic acid, measured as % TFAs.These strains over-express at least one .about.9 elongase/.about.8 desaturasemultizyme, in addition to other heterologous .about.9 elongases, .about.8desaturases, .about.5 desaturases, .about.17 desaturases, .about.12desaturases, C16/18 elongases, and optionally over-express diacylglycerolcholinephosphotransferases, malonyl CoA synthetases and/or acyl-CoAlysophospholipid acyltransferases. The expression of atleast one peroxisome biogenesis factor protein is down-regulated. Methods forproducing EPA within said host cells, oils obtainedfrom the cells, and products therefrom are claimed.