METHODS OF IDENTIFYING TARGET BINDING LIGANDS FROM TAGGED COMBINATORIAL LIBRARIES

摘要

Disclosed herein are methods of identifying target binding ligands from tagged combinatorial libraries. The methods generally involve contacting a combinatorial library with a target, washing the target to remove non-binding ligands, eluting ligands which bind to the target and identifying the ligands which were bound to the target. In some embodiments, the target may be a cell, tissues or membrane preparations. In other embodiments, the target may be attached to a matrix. In still other embodiments, the matrix may be packed in a column. The ligands may be operatively linked to monovalent oligonucleotides or multivalent oligonucleotides. Also, disclosed herein are various multivalent oligonucleotide architectures and methods of preparing and using combinatorial libraries which include multivalent oligonucleotides.