RIBOSOME DISPLAY COMPLEX AND PRODUCTION METHOD THEREFOR

摘要

The present invention addresses the problem of providing: a ribosome complex which can be prepared without involving a complicated reaction procedure, and in which a presented polypeptide is modified without impairing the function of a ribosome (particularly, the function for producing a polypeptide library); and a method for preparing the ribosome complex. The ribosome display complex production method according to the present invention is characterized by comprising: a step for translating an mRNA molecule using a cell-free peptide synthesis system utilizing a ribosome, to obtain a ribosome complex including an unmodified polypeptide chain, the mRNA molecule, and the ribosome; and a step for reacting a side-chain reactive functional group included in the unmodified polypeptide chain with a modification reagent to modify the unmodified polypeptide chain.