RIBOSOME DISPLAY COMPLEX AND PRODUCTION METHOD THEREFOR

摘要

The objective of the present invention is to provide a ribosome complex which can be produced without a cumbersome reaction procedure and of which displayed polypeptide is modified without impairing a function of the ribosome, particularly function to produce a polypeptide library, and a method for producing the ribosome complex. The method for producing a ribosome display complex according to the present invention is characterized in comprising the steps of translating the mRNA molecule in a cell-free peptide synthesis system utilizing the ribosome to obtain a ribosome complex comprising an unmodified polypeptide chain, the mRNA molecule and the ribosome, and modifying the unmodified polypeptide chain by reacting the side chain reactive functional group in the unmodified polypeptide chain with a modifying reagent.