LENTIVIRAL VECTOR FOR IMPROVING EXPRESSION LEVEL OF SELP GENE, AND APPLICATIONS THEREOF

摘要

A lentiviral expression vector for specifically promoting high expression of an SelP gene comprises a fundamental sequence, a resistance gene sequence, a multiple-cloning-sites sequence, a promoter sequence, and an SelP gene cDNA sequence of a pLVX-IRES-puro expression vector. The multiple cloning sites comprise an Spe I enzyme cutting site and an Not I enzyme cutting site, the SelP gene cDNA sequence comprises an Spe I enzyme cutting site, an SelP gene coding sequence and an Not I enzyme cutting site, and the SelP gene cDNA sequence is forwardly inserted into the multiple-cloning-sites sequence. The lentiviral expression vector has the advantages of high transfection efficiency and small usage and being capable of specifically, continuously, efficiently and stably expressing the SelP gene, and can serve as a powerful tool applied to the research and development of drugs related to SelP.