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METHOD FOR PREPARATION OF RECOMBINANT GLYCOPROTEIN HAVING N-LINKED GLYCAN ANTENNA STRUCTURE REINFORCED BY INHIBITION OF POLYLACTOSAMINE BIOSYNTHESIS
METHOD FOR PREPARATION OF RECOMBINANT GLYCOPROTEIN HAVING N-LINKED GLYCAN ANTENNA STRUCTURE REINFORCED BY INHIBITION OF POLYLACTOSAMINE BIOSYNTHESIS
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机译:具有通过抑制聚乳酸肌动蛋白生物合成而增强的N-连接甘氨酸天线结构的重组糖蛋白的制备方法
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摘要
In the present invention, it was confirmed that polylactosamine was reduced in recombinant human-derived erythropoietin produced by inhibiting (knocking down) the expression of the β3gnt2 gene, which is involved in polylactosamine biosynthesis, or from a cell line with the knocked out β3gnt2 gene, and thus triple and/or quadruple antenna structures of N-glycan was increased, leading to positive roles in increasing the half-life of glycoprotein and the efficacy of glycoprotein. Therefore, it was confirmed that a recombinant glycoprotein with an increased half-life can be produced through the method of the present invention.
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