首页>
外国专利>
N- Method for preparing recombinant glycoproteins with improved N-glycan antennary structure by inhibiting biosynthesis of polylactosamine
N- Method for preparing recombinant glycoproteins with improved N-glycan antennary structure by inhibiting biosynthesis of polylactosamine
展开▼
机译:N-通过抑制聚乳糖胺的生物合成来制备具有改善的N-聚糖触角结构的重组糖蛋白的方法
展开▼
页面导航
摘要
著录项
相似文献
摘要
In the present invention, it was confirmed that polylactosamine was reduced in recombinant human-derived erythropoietin produced by inhibiting (knocking down) the expression of the β3gnt2 gene, which is involved in polylactosamine biosynthesis, or from a cell line with the knocked out β3gnt2 gene, and thus triple and/or quadruple antenna structures of N-glycan increased, leading to positive roles in increasing the half-life of glycoprotein and the efficacy of glycoprotein. Therefore, it was confirmed that a recombinant glycoprotein with an increased half-life can be produced through the method of the present invention.
展开▼