STIMULATED EMISSION DEPLETION NONLINEAR STRUCTURED ILLUMINATION MICROSCOPY (STED-NSIM) APPARATUS, METHODS, AND APPLICATIONS

摘要

A superresolution STED-NSEVI apparatus having an epifluorescence architecture utilizing a 2D structured STED pattern having a N.A. less than a N.A. of the microscope objective and no surface plasmon resonance (SPR) effects. A superresolution STED-NSEVI imaging method using a fully deterministic imaging processing method, in which a pre- calibrated set of parameters are used to process all image data.