A lateral super-resolution imaging method using structured illumination without phase shift

摘要

Structured illumination microscopy has been a useful method for achieving lateral super-resolution,but it typically requires at least three precise phase shifts per orientation.In this paper,we propose a super-resolution method that utilizes structured illumination without phase shift.The reconstruction process requires only a conventionally illuminated image and an image with structured illumination.This method achieves the same effect as the traditional phase shift method,and more than doubles the resolution by synthesizing a few reconstructions at different illumination frequencies.We verify the resolution improvement process using a combination of theoretical derivations and diagrams,and demonstrate its effectiveness with numerical simulations.