PURIFICATION METHOD FOR PRIMARY TREATMENT OF CHO CELL CULTURE MEDIUM

摘要

A purification method for a primary treatment of a CHO cell culture medium is provided, comprising the following steps: 1) harvesting a CHO cell culture medium; 2) adding a flocculant, adjusting a pH value and stirring; and 3) clarifying the CHO cell culture medium from step 2). The purification method utilizes a combination method of chitosan, polyaluminum chloride and pH to flocculate cells, cell debris, HCP, DNA and other impurities in the cell culture medium. In addition, the residual chitosan and polyaluminum chloride can be further removed in a downstream process, which can the amount of final chitosan residue to less than 1 ppm.