CULTURE MEDIUM AND METHOD FOR IN VITRO CULTURING HUMAN ADULT PRIMARY MESENCHYMAL STEM CELLS ON A LARGE SCALE, PRIMARY MESENCHYMAL STEM CELLS OBTAINED BY THE METHOD, THE USES THEREOF

摘要

A method for in vitro preparing human adult primary mesenchymal stem cells (pMSCs, which are positive for Flk1 marker and Nanog marker) derived from human adult tissues (marrow) on a large scale and the products obtained by the same are provided. Uses of the method and the products obtained by the same in inhibiting the proliferation of tumor cells are also provided. By modifying the key processes and parameters of the preparation of pMSCs, for example simplifying the process of magnetic cell sorting, using human serum culture medium instead of bovine serum culture medium, and using the culture condition at low concentration oxygen (2%), the aim of preparing pMSCs on a large scale is achieved. By using the property of expressing Nanog gene of pMSCs, the novel use of pMSCs in inhibiting the proliferation of tumor cells is invented.