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SERUM-FREE MEDIUM INDUCING DIFFERENTIATION OF UMBILICAL CORD MESENCHYMAL STEM CELL INTO INSULIN-SECRETION-LIKE CELL AND PREPARATION METHOD AND USE THEREOF
SERUM-FREE MEDIUM INDUCING DIFFERENTIATION OF UMBILICAL CORD MESENCHYMAL STEM CELL INTO INSULIN-SECRETION-LIKE CELL AND PREPARATION METHOD AND USE THEREOF
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机译:无血清培养基诱导脐带间充质干细胞向胰岛素分泌样细胞的分化及其制备方法和用途
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摘要
Provided is a new type serum-free medium. The medium comprises: DMEM with high glucose (the content of glucose being 4.5 g/L), B27, recombinant human basic fibrolast growth factor (b-FGF), nicotinamide, N-2, vinblastine III (conophylline), non-essential amino acid (NEAA), heparin, epidermal growth factor (EGF), hepatocyte growth factor (HGF), a serum replacement (SR), an insulin-transferrin-selenium complex (ITS), and pentagastrin. Inducing differentiation of mesenchymal stem cells into insulin-secretion-like cells can be achieved in six days in one step using the medium.
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机译:提供了一种新型的无血清培养基。培养基包括:高葡萄糖DMEM(葡萄糖含量为4.5 g / L),B27,重组人碱性成纤维细胞生长因子(b-FGF),烟酰胺,N-2,长春碱III(茶碱),非必需氨基酸酸(NEAA),肝素,表皮生长因子(EGF),肝细胞生长因子(HGF),血清替代品(SR),胰岛素-转铁蛋白-硒复合物(ITS)和五肽胃泌素。使用该培养基,可以在一天内完成六天的间充质干细胞向胰岛素分泌样细胞的诱导分化。
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