FLUORESCENCE IMMUNOCHROMATOGRAPHIC DETECTION CARD AND PREPARATION METHOD THEREFOR AND USE THEREOF

摘要

Disclosed are a fluorescence immunochromatographic detection card and a preparation method therefor and the use thereof. The fluorescence immunochromatographic detection card comprises a treatment liquid A, a treatment liquid B, and a detection card. The treatment liquid A contains an antibody 1 that is coupled to a fluorescent microsphere and targets an antigen to be tested. The treatment liquid B contains an antibody 2 that is coupled to a biotin and targets an antigen to be tested. The detection card comprises a detection line area and a quality control line area, and a streptavidin detection T line is fixed in the detection line area, and an antibody quality control C line is fixed in the quality control line area. The preparation method comprises: (1) formulating the treatment liquid A; (2) formulating the treatment liquid B; and (3) lining on the detection card. The fluorescence immunochromatographic detection card has characteristics such as high sensitivity, high specificity, and high stability, and can be applied to the rapid detection of disease markers.