LIGATION-DEPENDENT PROBE AMPLIFICATION DETECTION METHOD FOR TRACE DNA

摘要

A ligation-dependent probe amplification detection method for trace DNA, comprising the following steps: (a) concentrating, or evaporating a sample of trace DNA to powders; (b) conducting a ligation reaction using the concentrated or evaporated DNA as a template, the ligation reaction being conducted in a ligation system of which the volume being no more than 20 μL and the amount of the template DNA being no more than 50 ng, and the ligation system being a micro ligation system which is treated taking a volatilization prevention step; (c) performing multiplex PCR amplification using the product of the ligation reaction as a template, such that an amplification product is obtained. Said method requires fewer amount of the template, that is, in said micro ligation system, a ligation effect of a conventional ligation system and high specificity and stability can be achieved even using the template DNA in a total amount of less than 50 ng. Moreover, said method consumes fewer reagents, that is, said micro ligation system has a much reduced volume compared to a conventional ligation system, and thus the reagent consumption of each component is reduced, saving reagent costs.