PREPARATION METHOD FOR HUMAN BREAST MILK STEM CELLS AND APPLICATION THEREOF

摘要

A preparation method for human breast milk stem cells and an application thereof. The preparation method comprises the following steps: collecting breast milk; separating human breast milk cells to obtained purified breast milk cell suspension; transferring the purified breast milk cell suspension into a culture plate coated with gelatin having a volume percentage of 0.01-0.05%, putting the culture plate into a CO₂ incubator for culturing, changing liquid once every day, separating individual cells on the sixth day for cloning and inoculating the cells into a new ultralow-adherency culture plate to promote clone formation; when the cells are fused to 80% or above, adding trypsin, performing digestion at 37°C for 5 min, ending the digestion, collecting cell sediments, washing and centrifugating the cells, resuspending the cells with a fresh cell culture liquid, and perform passage according to the ratio of 1:3. The technical solution is easy to operate, needs no co-culture of feeder cells, and can better maintain the undifferentiated state of the human breast milk stem cells and obtain a larger number of human breast milk stem cells.