WIDE-FIELD, MULTI-SCALE AND HIGH-RESOLUTION MICROIMAGING SYSTEM AND METHOD

摘要

A wide-field, multi-scale and high-resolution microimaging system (100), and a wide-field and high-resolution microimaging method. The wide-field, multi-scale and high-resolution microimaging system (100) comprises a light source (200), a sample (300), a microimaging objective (400), a collection system (700), and a workstation (1000). The microimaging objective (400) is used for imaging the sample (300) irradiated by light beams emitted by the light source (200) to obtain a wide-field curved image plane (410); the collection system (700) comprises a collection lens array (800) used for collecting the curved image planes (410) field by field and a camera array (900) used for secondary imaging; the workstation (1000) analyzes the imaging overlapping fields obtained by collection and obtains a final multi-scale, wide-field and high-resolution image by means of field splicing. By means of the design of the object len (400) and the improvement of an imaging method, multi-scale and high-resolution observation of cells, tissues and organs is achieved while ensuring a wide field range.