WIDE-FIELD MULTI-SCALE HIGH-RESOLUTION MICROSCOPIC IMAGING SYSTEM AND METHOD

摘要

The invention discloses a wide-field multi-scale high-resolution microscopic imaging system, which includes a light source, a sample, a microscope imaging objective system, an acquisition system, and a work-station. The microscope imaging objective system is used for forming an image after a light beam emitted by the light source illuminates the sample. To obtain a wide-field curved image planer, the acquisition system includes an acquisition lens array for subfield acquisition of the curved image plane, and a camera array for secondary imaging. The work-station is used for analyzing the acquired image in overlapping fields of view, and performing image splicing based on the fields of view, to finally obtain a wide-field high-resolution image. Because of the desire for an improvement to an imaging method, this invention realizes multi-scale high-resolution observation from cells, tissues, to organs while ensuring a wide field of view.