TUD RNA FOR ANTAGONIZING HUMAN MIRNA-29A, MIR-140 AND MIR-148A EXPRESSIONS, AND APPLICATION THEREOF

摘要

A Tud RNA for antagonizing human miRNA-29a, miR-140 and miR-148a expressions, and an application thereof. The DNA sequence of the Tud RNA for inhibiting human miRNA-29a, miR-140 and miR-148a expressions is shown by SEQ ID NO 1. A recombinant plasmid containing the Tud RNA and a preparation method therefor. The method comprises first designing the DNA sequence of the Tud RNA that antagonizes human miRNA-29a, miR-140 and miR-148a, cloning the DNA sequence onto a pENTR/U6 vector, and transferring the DNA sequence in a recombinant vector pENTR/U6-Tud-29a-140-148a to a lentivirus vector CS-RfA-EG by means of a Gateway technology so as to obtain a recombinant plasmid CS-RfA-EG-Tud-29a-140-148a. The recombinant plasmid can inhibit human miRNA-29a, miR-140 and miR-148a expressions.