METHOD AND TEST ELEMENT FOR ELECTROCHEMICALLY DETECTING AT LEAST ONE ANALYTE IN A SAMPLE OF A BODY FLUID

摘要

The present invention discloses a method and a test element 110 for electrochemically detecting at least one analyte in a sample 126 of body fluids comprising the steps of: a) providing at least one test element (110), wherein the test element (110) comprises at least one first electrode (112) in contact with the test chemical (122), and at least one second electrode Wherein the test chemical comprises an oxidized form of a redox mediator or a redox mediator of the oxidized form and wherein in the absence of the sample (126), a sample of the body fluid 126), wherein the surface (120) of the second electrode (114) is comprised of a silver metal; providing the at least one test element (110); b) contacting both the first electrode 112 and the second electrode 114 with a sample 126 of body fluids comprising chloride ions; c) between the first electrode 112 as a cathode and the second electrode 114 as an anode for a predetermined time and at a voltage sufficient to form a silver chloride layer on the surface 120 of the second electrode 114; Applying a first voltage (V ₁ ), wherein the oxidized form of the redox mediator is converted to a reduced form; applying the first voltage (V ₁ ); d) applying a second voltage (V ₂ ) between the first electrode (112) as the anode and the second electrode (114) as the cathode; And e) determining an electrical signal between the first electrode (112) and the second electrode (114), wherein the analyzing in the sample (126) of the bodily fluid containing chloride ions And detecting the analyte comprises the step of re-oxidizing the redox mediator. The invention also includes a method for fabricating a test element 110, a method for creating a silver chloride layer on the electrode surface of the test element 110, a system for determining at least one characteristic of a body fluid sample 126, Wherein the system includes a test element (110).