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METHOD AND TEST ELEMENT FOR ELECTROCHEMICALLY DETECTING AT LEAST ONE ANALYTE IN A SAMPLE OF A BODY FLUID
METHOD AND TEST ELEMENT FOR ELECTROCHEMICALLY DETECTING AT LEAST ONE ANALYTE IN A SAMPLE OF A BODY FLUID
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机译:体液样品中至少一种分析物的电化学检测方法和试验元素
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摘要
The present invention discloses a method and test element 110 for electrochemically detecting at least one analyte in a sample 126 of body fluid, the method comprising the following steps: a) providing at least one test element 110, wherein the test element 110 comprises at least one first electrode 112 in contact with a test chemical 122, and at least one second electrode ( 114), wherein the test chemical 122 is a redox mediator in an oxidized form or comprises a redox mediator in an oxidized form, and in the absence of the sample 126, a sample of the bodily fluid ( 126) providing the at least one test element (110), wherein the surface (120) of the second electrode (114) is configured from silver metal; b) contacting both the first electrode 112 and the second electrode 114 with a sample 126 of the bodily fluid containing chloride ions; c) between the first electrode 112 as a cathode and the second electrode 114 as an anode, for a period of time and at a voltage sufficient to form a layer of silver chloride on the surface 120 of the second electrode 114. Applying a first voltage (V 1 ), wherein the oxidized form of the redox mediator is converted to a reduced form, applying the first voltage (V 1 ); d) applying a second voltage (V 2 ) between the first electrode 112 as an anode and the second electrode 114 as a cathode; And e) determining an electrical signal between the first electrode 112 and the second electrode 114, wherein the analyte in the sample 126 of the bodily fluid containing chloride ions is determined by the determining step Detecting, and detecting the analyte includes determining the electrical signal, comprising reoxidizing the redox mediator. The present invention also provides a method for manufacturing a test element 110, a method for producing a layer of silver chloride on the electrode surface of the test element 110, and a system for determining at least one property of a sample 126 of bodily fluid. Disclosed herein, the system includes a test element 110.
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