- Cell spheroid formed by co-culture of human gingiva-derived stem cell and osteoprecursor cell and increasing method of VEGF production thereof

摘要

The present invention relates to a method for increasing production of vascular endothelial growth factors (VEGFs) in stem cells by co-culturing gum-derived stem cells and osteoprecursor cells, a composition for promoting osseointegration or a composition for treating ischemic diseases comprising the vascular endothelial growth factor as an active ingredient, and a method for forming a cell spheroid by co-culturing gum-derived stem cells and osteoprecursor cells. According to the present invention, by co-culturing the gum-derived mesenchymal stem cells and the osteoprecursor cells together, it is possible to increase yield of stem cell-derived VEGF, and production of the cell spheroid is possible by the co-culture. In addition, by using the composition comprising, as the active ingredient, a culture solution with enhanced VEGF content from the cell spheroid produced by the present invention, it is possible to effectively treat osteopathy by promoting osseointegration in the teeth, and to effectively treat ischemic diseases by stimulating angiogenesis.