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p60 p80 katanin p60 protein and katanin p80 protein recombinant vector comprising the gene coding the protein cells transformed by the vector and preparing method for modified katanin protein using the same
p60 p80 katanin p60 protein and katanin p80 protein recombinant vector comprising the gene coding the protein cells transformed by the vector and preparing method for modified katanin protein using the same
The present invention relates to a method for producing katanin proteins in bulk, by inducing expression in Escherichia coli so as to keep a three-dimensional structure of human katanin protein stable and intact. To this end, a gene on a portion three-dimensionally bindable in a p60 protein and a p80 protein which make up the human katanin protein is found, enabling mass-production of water-soluble and three-dimensional katanin proteins using the same. The water-soluble and three-dimensional katanin protein obtained thereby is anticipated to play a crucial role in regulating microtubules.
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