A spontaneously beating cardioorganoid structure and an integrated body-on-chip device containing the same

摘要

A method of making a heart structure comprises depositing a mixture comprising living mammalian cardiac cells (e.g., individual cells, organogens or spoloids), fibrinogen, gelatin, and water on a support to form a middle heart structure step; Optionally co-depositing a structural support material (e. G., Polycaprolactone) with the mixture in the form of supporting the intermediate structure; And then an effective amount of the fibrinogen to produce a heart structure that is composed of live heart cells that are crosslinked and spontaneously pulsate together in the fibrin hydrogel (with intermediate incubation, depending on the maturation of the starting cardiac cells, if necessary) Contacting the construct with thrombin. The generated products and how to use them are also explained.;