-cell-free DNA Magnetic nanostructure for detecting and isolating circulating cell-free DNA comprising conductive polymers containing magnetic nanoparticles and cationic polymers

摘要

The present invention relates to a magnetic nanostructure for detecting and isolating cell-free DNA comprising a cationic polymer and a conductive polymer containing magnetic nanoparticles. The magnetic nanostructure for detecting and isolating cell-free DNA, comprising a cationic polymer and a conductive polymer containing magnetic nanoparticles, according to the present invention, can efficiently detect various types of cfDNA from a small amount of a biological sample such as urine, cerebrospinal fluid, blood plasma, blood or body fluid. In particular, due to a strong magnetic field generated from a large amount of magnetic nanoparticles included in the magnetic nanostructure, the magnetic nanostructure can efficiently capture cfDNA present in a trace amount in a sample. Also, in detecting, isolating, and extracting cfDNA, by having a long structure of the magnetic nanowire structure, the magnetic nanostructure can have a large amount of cationic polymers, polyethylenimine, attached thereto, and thus can greatly increase the efficiency of capturing DNA present in a sample. Therefore, the use of the magnetic nanostructure results in enhanced detection sensitivity, which leads to significantly improved cfDNA detection and isolation as compared to the conventional art. Also, by having a thread-like thin and long structure, the magnetic nanowire can effectively detect and capture cancer-related cfDNA by penetrating through gaps among a large number of cells or proteins present in blood or urine, and thus exhibits a significantly improved detection efficiency. Therefore, it is expected that the magnetic nanostructure for detecting and isolating cfDNA according to the present invention will be used for extracting DNA from blood cancer cells for use in a genetic mutation diagnosis service, as well as for an early cancer diagnosis and cancer treatment.