high-throughput screening method for measuring the activity of gap junctional intercellular communication

摘要

The present invention relates to a high-speed screening method for measuring a gap junction activity between a donor cell expressing an iodide transporter and a receptor cell expressing an iodide sensor fluorescent protein, (iodide) transporter membrane protein and iodide sensor YFP ^QL protein is co-cultured with iodide, iodide enters the donor cell and passes through the gap junction to the receptor cell And the transferred iodide can bind to the YFP ^QL protein to quench the fluorescence and it can be confirmed that the change in the activity of the gap junction can be sensitively and quickly measured by measuring the iodide. A method for measuring the intercellular gap junction activity of a drug screening chamber using a gap junction activity regulator To be useful. In addition, since the method of measuring the gap junction activity of the present invention can be applied to a cell expressing a connexin protein which is a tube protein forming a gap junction, it has a wide range of application fields and a Z 'value (Z'factor ), It is effective as an ultrafast screening analysis.