UnaG method for detecting subcellular bilirubin by UnaG protein

摘要

The present invention includes the steps of transforming the living cells, to a method for detecting bilirubin present in organelles, cell organelles target polynucleotide and UnaG cell with a recombinant vector comprising a poly nucleotide tie encoding the protein, transformants the step of measuring the fluorescence intensity of UnaG protein in the cell, transfection step of measuring the expression level of UnaG protein in the transformed cells, and by comparing the degree of fluorescence intensity and the expression including the steps to determine the coefficient of determination, and trends cells relates to my bilirubin DETERMINATION.;