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Recombinant vector containing cellulose binding domain and Purification method for a protein using thereof

机译:含有纤维素结合结构域的重组载体及其蛋白纯化方法

摘要

The present invention relates to a recombinant vector comprising a cellulolytic domain 3 and to a method for separating a target protein using the recombinant vector, wherein the method for separating the protein of the present invention binds the plant transformed with the recombinant vector to cellulose to easily isolate a fusion protein containing the target protein, and treats the fusion protein with enterokinase to efficiently isolate the target protein from the cell binding domain, thereby being expected to be applicable to various industrial fields.
机译:本发明涉及包含纤维素分解结构域3的重组载体和使用该重组载体分离靶蛋白的方法,其中本发明的分离蛋白的方法将用重组载体转化的植物与纤维素结合至纤维素。分离含有靶蛋白的融合蛋白,并用肠激酶处理融合蛋白以有效地从细胞结合域分离靶蛋白,从而有望应用于各种工业领域。

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