Novel sequence of cellulose binding domain recombinant expression vector composed with the same and protein purification method for a protein using thereof

摘要

The present invention relates to a novel nucleotide sequence of a cellulose binding domain, a recombinant vector comprising the same, and a method for separating and purifying proteins using the same. More specifically, the present invention relates to a xylase 10A derived from Thermo Thermoga maritima. A cellulose binding domain sequence encoding a cellulose binding domain CBM9 (N38Q) in which asparagine, a 38th amino acid of the CBM9 (family 9 carbohydrate-binding module) domain of a protein, is substituted with glutamine; A recombinant vector comprising the cellulose binding domain nucleotide sequence; Cellulose comprising the amino acid sequence of the cellulose binding domain CBM9 (N38Q) substituted with glutamine substituted asparagine, the 38th amino acid of the family 9 carbohydrate-binding module (CBM9) domain of the xylanase 10A protein from Thermotoga maritima Peptides of binding domains; And it relates to a method for separating proteins comprising such a cellulose binding domain (Cellulose binding domain).