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Method for Purifying Human Serum Albumin from Transgenic Rice Grain

机译:从转基因水稻籽粒中纯化人血清白蛋白的方法

摘要

The present invention provides a method for purifying human serum albumin from transformed rice grains, comprising the steps of: 1) performing a cation exchange chromatography on the crude extract of rHSA to obtain a first product I; 2) performing anion exchange chromatography on the primary product I to obtain a secondary product II; 3) Performing hydrophobic chromatography on the secondary product II to obtain purified rHSA. The method may further comprise the step of ceramic hydroxyapatite chromatography prior to hydrophobic chromatography.
机译:本发明提供了一种从转化的米粒中纯化人血清白蛋白的方法,其包括以下步骤:1)对rHSA的粗提物进行阳离子交换色谱法以获得第一产物I; 2)对一级产物I进行阴离子交换色谱,得到二级产物II; 3)对副产物II进行疏水层析,得到纯化的rHSA。该方法可以进一步包括在疏水色谱之前进行陶瓷羟基磷灰石色谱的步骤。

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