Cre Process for preparing exosome loading cre recombinase and pharmaceutical composition for use in generating conditional knock-out alleles containing the same as an active ingredient

摘要

The present invention relates to a method for producing a large amount of exocom (Cre: EXPLOR) containing a cre protein (cre recombinase), a vector for producing an exosome that can be used for producing the exocom, a cre recombinase produced by the method, And a composition for producing a conditional knockout allele of a target gene containing the same as an active ingredient. Since the exosome containing the cre protein (cre recombinase) can be produced with a high yield by using the method for producing exosome including the cre recombinase provided by the present invention, Can be widely used as a composition for producing a conditional knockout allele of a gene.