High resolution detection of DNA abasic sites

摘要

The present invention provides method of labelling a nucleic acid containing an abasic (AP) site. The method includes the step of reacting a nucleic acid comprising an AP site with an aldehyde probe, such that the probe covalently binds to the AP site to produce a nucleic acid labelled with the probe; optionally oxidising the nucleic acid labelled with the probe; optionally isolating the labelled nucleic acid, and selectively cleaving the nucleic acid at the AP site to produce nucleic acid fragments.